P-375 Effect of D-Chiro-Inositol in a mouse model of endometriosis

Abstract Study question Can D-Chiro Inositol administration mitigate the phenotype of endometriosis in a mouse model? Summary answer Based on an model, we demonstrated that can reduce development endometriotic lesions. What is known already Endometriosis, disease affecting 5-10% women reproductive age, characterized by spread endometrial-like tissue outside uterine cavity produces ectopic lesions causing pain and infertility. The sensitivity to estrogens characteristic be used for therapeutic purposes. (DCI), one nine isomers Inositol, decrease CYP19A1 aromatase gene expression granulosa cells. these premises, it was suggested treatment with DCI may have clinical application conditions where decreased estrogen levels required. design, size, duration To address study question, model generated. Out 20 CD1 mice, 4 mice were randomly selected as donors fragments remaining 16 recipient mice. first day after transplantation, assigned four experimental group which received 28 days 2ml water containing: none (CTRL); 0.4mg (DCI 0.4); 0.2mg Dienogest 0.33ng 0.2+DG 0.33); DG 0.67ng (DG 0.67). Participants/materials, setting, methods Uterine horns removed from donor at diestrous stage cycle. cut into inoculated intraperitoneal injection. Four weeks induction, all sacrificed. Their excised, measured number examined presence blood vessels vascularization under stereomicroscope. Then, processed histology examination hematoxilin-eosin (H&E) Azan Mallory staining. Main results role chance Endometriotic developed met criteria endometriosis, including endometrial epithelial stromal cells, encapsulation neighboring tissues or organs. reduced groups when compared control (p < 0.05, t-test), no differences observed among 0.4, 0.33 0.67. Concomitantly rate vascularized lower treated groups, more pronounced effect 0.4 t-test). histological analysis revealed marked reduction foci groups. These provide evidence not employed dose association DG. Although molecular mechanisms underlying effects requires further investigation, present findings support hypothesis could effective than mitigating phenotype. Limitations, reasons caution Results animal studies should extrapolated humans caution. Wider implications Present open new avenue testing whether therapy. Trial registration Not Applicable